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Biological Scientist III

Employer
University of Florida
Location
Florida, United States
Salary
Salary Not specified
Date posted
Dec 22, 2021

Perform calculations, design and implementation of laboratory techniques, maintain records and analyze results. Culture cell lines, determine cytokine titers, test insulin secretion and perform flow cytometry analysis. Manipulate protein reagents for use in immunoassay; perform qRT-PCR, ELISA’s, immunofluorescence; protein putrification, maintain antibodies and protein standards for Western and SDS-PAGE protocols.

 

Develop new assays and methodologies as needed. Serve to maintain quality control for assays performed throughout the laboratory; troubleshoots assays that do not perform well. Helps to create, document, organize and modify formal SOPs (standard operating protocols/procedures) based on cGLP (current good laboratory practices) guidelines. Performs QA/QC procedures for all scientific procedures performed and creates QA/QC procedures for new scientific protocols.

 

Data analysis and documentation. The candidate should contribute with all the general laboratory duties, cleaning, maintenance of cell lines stocks, chemical stocks etc

 

Perform animal studies. Perform testing of natural and synthetic nanoparticles on rats and mice.

 

Coordinate the clinical trials for assessing the use of Lactobacillus johnsonii in patients with type I diabetes. Duties include but are not limited to subject recruitment, preparation of documents for clinical trial participants according to guidelines set forth by IRB, oversee blood and stool sample processing, coordinate blood sample testing for participants (immunophenotyping, c-peptide and autoantibody analysis), assemble stool collection kits, coordinate shipping, receiving and processing of stool samples from enrolled subjects throughout study, assign randomized treatments for enrolled subjects, ship assigned treatments to enrolled subjects throughout study, and attend diabetes-related events in central Florida to facilitate subject recruitment. Prepare capsules required to conduct the clinical trials. Duties include contacting CMO/CDMOs that meet FDA CGMP requirements for production of clinical trial products, obtain quotes, facilitate vendor approval, coordinate delivery of raw materials (milk powder, L. johnsonii stocks) to fermentation company, coordinate delivery of the lyophilized probiotic to the manufacturing company for encapsulation and packaging, coordinate testing of finished probiotic and placebo products for USP and USP compliance, conduct monthly testing of final products (CFU and contamination analysis).

 

Field of Study

Microbiology

 

 

Minimum Requirements:             

Bachelor’s degree in biology or a closely related field and seven years of relevant experience; or a Master’s degree in biology or a closely related field and five years of relevant experience; Doctoral degree in biology or a closely related field and two years of relevant experience.

 

Those interested should apply by sending an email to Mary Jane Lukas, m.lukas@ufl.edu

 

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