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Research Technologist

Employer
Johns Hopkins University
Location
Maryland, United States
Salary
Salary Not Specified
Posted Date
Oct 20, 2021

View more

Position Type
Faculty Positions, Science, Technology & Mathematics, Biotechnology & Bioengineering, Administrative, Academic Affairs, Research Administration
Employment Type
Full Time
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The Sidney Kimmel Cancer Center at Johns Hopkins is seeking a Research Technologist who will become a member of a cutting-edge academic laboratory studying the cellular metabolism of immune cells, particularly as it relates to the immune response to cancer. The overarching goal of the Leone lab is to elucidate fundamental cellular processes (e.g., differentiation) activated in response to metabolic events that have broad implications for development of immune-based therapeutics in cancer and autoimmune disease. We employ an extensive range of immunologic models in mice, in vitro studies, as well as patient samples.

The applicant will be responsible for performing experiments using basic and emerging immunological techniques, combined with state-of-the-art technologies for assessing cellular metabolism. They will engage in innovative discovery projects and assist with general laboratory tasks, including processing of human/mouse tissue specimens, cell culture, and general maintenance of laboratory equipment and supplies.

Specific Duties & Responsibilities:

    Set up and maintain in vitro cell cultures Analyze data and interpret findings Follow established lab procedures and protocols and utilize aseptic technique to perform experiments, including: basic tissue culture, cell separation methods, protein and DNA electrophoretic separations, qRT-PCR assays, flow cytometry, CRIPR-Cas9 genome editing techniques, preparing samples for mass spectrometry and other biological techniques Will utilize mouse models in experiments Maintain accurate, detailed data records in laboratory notebooks and electronically on file servers, ensuring their appropriate file backup, description, accuracy, and completion Must be able to communicate well and work with others Assist with research projects, published journal database searches, manuscript preparation and troubleshooting, as needed Participate in general laboratory tasks, including maintenance of lab equipment, monitoring inventory levels, and ordering materials and supplies in accordance with established policies and procedures Organize and manage orders of reagents and supplies Prepare reports of findings by compiling/annotating results, and preparing figures/tables, when required Presenting data to lab members at monthly meetings Prioritize daily tasks and responsibilities appropriately

Minimum Qualifications (Mandatory):

    BS/BA, or equivalent, in Biological Sciences, Chemistry, or related field required Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula
  • Six months of post-undergraduate experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements
  • Experience and knowledge of immunology or cancer a plus

Special Knowledge, Skills & Abilities:

  • Critical thinking and attention to detail is essential
  • Candidate should have some familiarity with the theory behind experiments to facilitate interpretation in relation to positive and negative controls
  • Experience with experimental design principles and reproducible research
  • Demonstrates analytic, planning, and organizational skills needed to manage multiple projects or responsibilities
  • Ideal candidate will possess experience cultivating cell lines, in vivo tumor models, quantitative real-time PCR, flow cytometry, mouse genotyping, western blotting, among other techniques.
  • Ideal candidate will possess experience with the operation of laboratory equipment such as PCR machines, real-time PCR machines, centrifuge, pH meter, analytical balance, incubator, spectrophotometer, light microscope, imaging systems and laminar flow hoods.
  • Ideal candidate would have some familiarity with immunology and have experience with flow cytometry, T cell culture, and cellular metabolism
  • Skilled in techniques of molecular biology (e.g. genome editing, overexpression) and generating high-throughput data (e.g., RNA-seq, ChIP-seq) would be highly desirable
  • Experience with data analysis using R programming and experience with CRISPR-Cas9 screens would be ideal, though not required
  • Other skills: Strong communication skills, comfortable working in a scientific team, and conversant in Microsoft office and Graphpad

Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.00 - $20.00/hr (commensurate with experience)
Employee group: Full Time
Schedule: M-F 9am-5pm
Exempt Status: Non-Exempt
Location: School of Medicine Campus
Department name: ​​​​​​​SOM Onc Cancer Immunology Lab Research
Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons or because the individual is pregnant or attempting to become pregnant. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit https://www.hopkinsmedicine.org/coronavirus/covid-19-vaccine/ and all other JHU applicants should visit https://covidinfo.jhu.edu/health-safety/covid-vaccination-information/.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer
Note: Job Postings are updated daily and remain online until filled.

EEO is the Law
Learn more:
https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf


The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer
Note: Job Postings are updated daily and remain online until filled.

EEO is the Law
Learn more:
https://www1.eeoc.gov/employers/upload/eeoc_self_print_poster.pdf
Important legal information
http://hrnt.jhu.edu/legal.cfm
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