Research Technologist

Johns Hopkins, founded in 1876, is America's first research university and home to nine world-class academic divisions working together as one university.

We are seeking a highly motivated, energetic Research Technologist with an interest in biomedical research to work in the field of Retinal Regenerative Medicine. We use stem cell biology to study human neuronal and glial cells. We use CRISPR genome editing to develop models of: 1) human retinal diseases, with a focus on neurodegenerative diseases such as glaucoma, 2) demyelinating diseases (such as multiple sclerosis where myelin sheath on the CNS neurons is lost), 3) Neurofibromatosis 1 (NF1), with a focus on the genetic mutations in NF1 gene that cause dysfunction of glial cells such as astroglia, oligodendroglia and microglia. We also use mouse model of neurodegenerative diseases for drug testing.

Specific Duties & Responsibilities

• This work will involve a considerable amount of cell culture and molecular biology work.
• The successful applicant will work under the supervision of the principal investigator and other laboratory personnel, and will be responsible for,
• Maintaining human pluripotent stem cells, as well as differentiating them into human retinal ganglion cells, oligodendrocytes, astrocytes, and microglia.
• Assist in various molecular biology work that include molecular cloning, DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and Western Blotting etc.
• While the majority of the work is related to cell culture and molecular biology, some animal handling and tissue processing will also be required, which they will need to perform under the supervision of the principal investigator or a senior scientist.

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry or related field.

Preferred Qualifications

• Some relevant post-undergraduate work experience in the field of cellular and molecular biology is desirable.
• Experience with cell culture work, ideally with human stem cell culture, is desirable.
• Prior experience handling and working with animal models is a plus.

Classified Title: Research Technologist
Role/Level/Range: ACRO40/E/02/CC
Starting Salary Range: $15.70 - $26.25 HRLY (Commensurate with experience)
Employee group: Full Time
Schedule: 40 hrs per week, Mon-Fri (some weekend hours required)
Exempt Status: Non-Exempt
Location: School of Medicine Campus
Department name: ​​​​​​​SOM Oph Ocular Molecular Biology
Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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