Research Technologist

The Department of Oncology seeks aResearch Technologistto work under the general supervision of a faculty investigator or post-doctoral fellow as well as semi-independent work, is responsible for laboratory research using established procedures and protocols. The position requires a basic understanding of molecular techniques required to carry out laboratory experiments, the ability to apply basic analytical skills to implement assigned projects and identify situations needing special attention.

The research focus of the laboratory is on the assembly and morphogenesis of herpesviruses. Using recombinant baculovirus technology to produce virus-like particles for structural studies and proteomic analysis. Creating recombinant viruses tagged with fluorescent reporters (GFP) to visualize in real-time virus particle movement within cells and in neurons. New projects also focus on development of protein microarrays using a virion display method for human transmembrane proteins. In addition, in conjunction with the Synthetic Biology team at JCVI we are synthetic biology methods to assemble the large genomes of herpesviruses.

DUTIES AND RESPONSIBILITIES

• Uses molecular biology methods (cloning, PCR assays, and mutagenesis), cell culture methods (transfections, virus infections, and cell line maintenance), biochemical procedures (protein electrophoresis, western blotting, immunoprecipitation, protein purification, protein microarray, and immunofluorescence), recombinant herpesvirus and baculovirus generation.
• Applies analytical skills to implement assigned projects
• Maintains accurate and current data records.
• Organizes and records plasmid constructs and stocks.
• Maintains freezer stock and supply inventory.
• Responsible for maintenance of lab and equipment.
• Identifies and trouble-shoots methods and experimental procedures.
• Exercises independent judgment.
• Good oral and written communication skills are required.

MINIMUM QUALIFICATIONS:

Bachelor’s Degree in biological sciences, chemistry or related field.

Preferred Experience: Some related post-undergraduate experience in a comparable laboratory, or a significant independent research project, not related to normal course requirements preferred.

Additional Knowledge, Skills, or Abilities / Competencies:

• Western blotting, PCR, cell culture

Equipment, Machine, or Tool Requirements:

• Centrifuges, pH meter, analytical balance, pipettes, spectrophotometer, fluorometer, electrophoresis equipment, digital photography equipment, and DNA thermal cyclers.
• General computer skills, with experience with Microsoft Office

Supervision of Others: Will supervise undergraduate students.

Physical Requirements:

Sitting, standing and walking for extended period. Reaching by extending hand(s) or arm(s) in any direction. Finger dexterity required to manipulate objects with fingers rather than with whole hand(s) or arm(s). Ability to move standard equipment through a hospital or laboratory environment.

Classified Title:Research Technologist
Role/Level/Range:ACRO40/E/02/CC
Starting Salary Range:$15 - $19.44/Hour; Commensurate with experience
Employee Group:Full Time
Schedule:Monday - Friday, 8:00am - 4:30pm
Exempt Status: Non-Exempt
Location:School of Medicine, East Baltimore Campus
Department Name: Oncology Viral Oncology
Personnel Area:School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office atjhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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