Research Specialist II

This position requires a candidate that can perform independent research involved in a major research project involving the study of skin regeneration and stem cells. This involves study of various differentin vivoandin vitroinfection and wounding models. The candidate will be responsible for performing various experimental procedures that include a working knowledge of microbiology, immunology and molecular biology techniques.

The experimental procedures include:in vivomodels transgenic and knockout mouse models that utilizein vivobioluminescence and fluorescence imaging, human organotypic cell cultures, various other human and mouse cell cultures, isolation of mouse and human cells from peripheral blood, isolation of mouse and human cells from the skin, immunohistochemistry, immunoassays (ELISA, myeloperoxidase assays), molecular biology techniques such as isolation of RNA, RT-PCR, real-time PCR, gel electrophoresis, affinity column purification, FACS/Flow cytometry, intracellular staining, and protein analysis and quantification (e.g. western blots). Experience with siRNA and gene viral transfection of cell culture models is useful.

The individual will also be responsible for a collaborative multidisciplinary project with a pharmacology lab on small molecule drug development. This will involve communicating with the pharmacology group, attending meetings, designing experiments, and executing in vitro and in vivo tests of potential small molecule hits. Also generally testing for agents that promote or inhibit hair growth.

In addition, this position will involve transfecting cells with various constructs to enable cells to overexpress or inhibit expression of various factors involved in the regeneration. This position will also involve the development of various different therapeutic strategies to help promote regeneration.

Classified Title:Research Specialist II
Working Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range:$38,920 - $53,520; Commensurate with Experience
Employee group: Full Time
Schedule: Monday-Friday, 8:30am-5:00pm, Up to 37.5 hrs/week
Exempt Status: Exempt
Location: 04-MD:School of Medicine Campus
Department name: 10002833-SOM Der Research Division
Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office atjhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer
Note: Job Postings are updated daily and remain online until filled.

EEO is the Law
Learn more:
https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf