Research Assistant II

Job Purpose

The Center for Cell and Gene Therapy to assist with testing and characterization of engineered CAR T-cell products for patients in Phase I clinical trials. Applicants are sought to perform assays for correlative CAR T-cell studies and conduct release testing on GMP-manufactured T-cell lines. Applicants are also expected to assist with lab organization and ordering.

Job Duties

• Optimize and perform under supervision cell-based assays for high-throughput analyses of CAR T-cells (multicolor flow cytometry, IsoPlexis, RNA-seq).
• Perform pilot experiments to test specific hypothesis in preclinical CAR-T cell development.
• Coordinate with CAGT GMP and GLP cores obtaining and processing primary patient samples collected at follow-up for subsequent analysis.
• Participate in validating new SOPs and worksheets for GMP manufacturing.
• Perform release testing of CAR T-cell lines manufactured in GMP such as standard cytotoxicity assays and flow cytometric analysis of patient's starting cell material, in-process CAR T-cells and final CAR T-cell products.
• Assist with preparing and infusing CAR T-cell lines at TCH and HMH (optional).
• Complete required paperwork in a timely manner.
• Maintain stock of reagents and log experimental details and results to electronic notebook.
• Assist with writing reports and manuscripts and present at scientific meetings.
• Maintain a list of common reagents required for correlative and research studies, restock in a timely manner.
• Collect and place orders for a laboratory (twice a week).
• Assist with obtaining quotes for research equipment and supplies.

Minimum Qualifications

• Bachelor's degree in a Basic Science or a related field.
• Three years of relevant experience.

Preferred Qualifications

• Master's degree in cell biology or related field.
• Three years of relevant experience in cell biology.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

5862

CA; CH