Research Technologist

Location
Maryland, United States
Salary
Salary Not Specified
Posted
Nov 06, 2020
Employment Type
Full Time

Research Technologist needed to manage daily operations of research laboratory investigating the dynamics of microbial pathogen exposures, immune responses, and human health. Position will support research projects to investigate environmental and occupational exposures to microbial pathogens and human health outcomes. The primary focus of the lab is development of novel, non-invasive assays to assess exposure to and infection with SARS-CoV-2 and human endemic coronaviruses, influenza and other respiratory viruses,S. aureus, and diverse enteric pathogens. The lab applies these novel assays in population-based studies of microbial exposure, infection, and immunity. Work requires use of scientific methods, procedures and techniques; though training will be provided, previous experience and a profound understanding of theory behind experiments to perform data analysis and troubleshoot situations that need special attention with a substantial degree of independence are highly preferred.

Specific Duties/Responsibilities:

  • Perform isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
  • Process biospecimens from community-based and clinical study populations.
  • Process saliva, anterior nares, serum, and plasma specimens for SARS-CoV-2 molecular and serological testing.
  • Perform nucleic acid extraction on saliva, anterior nares, serum, and plasma.
  • Test biospecimens from community-based and clinical study populations for SARS-CoV-2, influenza viruses, and other microbial pathogens.
  • Perform real-time PCR assays, Luminex microsphere magnetic bead-based immunoassays, ELISA.
  • Perform bacterial culture of environmental, human and animal specimens.
  • Testing of bacterial isolates for antimicrobial susceptibility. (disk diffusion)
  • Perform nucleic acid extraction. (manual and automated)
  • Perform RT-qPCR and endpoint PCR including polyacrylamide and agarose gel electrophoresis.
  • Prepare and test specimens for antibodies against pathogens and for pathogens. (ELISA; Luminex)
  • Maintain detailed records of quality assurance and quality control for specimens and specimen analyses.
  • Record and analyze results, organize data, perform basic computations, and develop reports and summaries of analyses.
  • Operate basic and advanced laboratory equipment, including automated nucleic acid extraction system, RT-qPCR instrument, microplate washer and reader, incubator, spectrophotometer, laminar flow hood, thermal cycler and other basic lab equipment.

Other responsibilities include:

  • Contribute to general maintenance of the laboratory, equipment and supplies.
  • Maintain and make minor adjustments to equipment, e.g. calibrations.
  • Monitor inventory levels, order materials and supplies in accordance with established policies and maintain records.
Minimum Qualifications:

  • Bachelor’s degree in fields related to biology, chemistry required.

Preferred Qualifications:

  • Some related post-undergraduate work experience preferred.

Special Knowledge, Skills, and Abilities:

  • Bacterial culture, isolation of bacteria and viruses, nucleic acid extraction, real time PCR, ELISA, Luminex multiplex immunoassays, cloning.
  • Equipment to be used: Nucleic acid extraction & qPCR instrument, Luminex instrument, ELISA reader.

Technical qualifications or specialized certifications:

  • CITI human subjects research ethics training, biosafety training, blood-borne pathogens training, IATA shipping training.
  • Any specific physical requirements for the job: In-person work in active BSL2 microbiology and immunology laboratory.
Classified title: Research Technologist

Working title: Research Technologist

Role/Level/Range: ACRO 40/E/02/CC

Starting Salary Range: $14.14 - $19.44 (commensurate with experience)

Employee group: Full time

Employee Status: Non-Exempt

Schedule: Monday-Friday - 8:30am-5:00pm 40hrs/Wk -

Location: School of Public Health, Rm E6649 – 615 N Wolfe St., Baltimore, MD

Department name:10001082-Environmental Health and Engineering

Personnel area (School): BSPH – Bloomberg School of Public Health

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office atjhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer
Note: Job Postings are updated daily and remain online until filled.

EEO is the Law
Learn more:
https://www.eeoc.gov/sites/default/files/migrated_files/employers/poster_screen_reader_optimized.pdf

School of Public Health - East Baltimore Campus


The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at jhurecruitment@jhu.edu. For TTY users, call via Maryland Relay or dial 711.

The following additional provisions may apply depending on which campus you will work. Your recruiter will advise accordingly.

During the Influenza ("the flu") season, as a condition of employment, The Johns Hopkins Institutions require all employees who provide ongoing services to patients or work in patient care or clinical care areas to have an annual influenza vaccination or possess an approved medical or religious exception. Failure to meet this requirement may result in termination of employment.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

Equal Opportunity Employer
Note: Job Postings are updated daily and remain online until filled.

EEO is the Law
Learn more:
https://www1.eeoc.gov/employers/upload/eeoc_self_print_poster.pdf
Important legal information
http://hrnt.jhu.edu/legal.cfm

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